rabbit polyclonal anti npc1l1  (Novus Biologicals)

Journal: Iranian Journal of Medical Sciences

Article Title: Effects of Saponin from Trigonella Foenum-Graecum Seeds on Dyslipidemia

doi:

Figure Lengend Snippet: ABCG8 and NPC1L1 protein expression in intestine. (2A) The protein bands of ABCG8, NPC1L1 and beta-actin. (2B) The data of the protein bands semi-quantitatively analyzed by QuantityOne. Data are reported as mean±SD of six rats carried out in duplicate. P values are obtained from the independent sample t-test between two groups (HFD group and another group). *P<0.05: Indicate significant difference from the HFD group; **P<0.01: Indicate extremely significant difference from the HFD group; ABCG8: ATP-binding cassette transporter G8, NPC1L1: Niemann-Pick C1-Like 1

Article Snippet: Membranes were cut into strips and blocked by 5% skim milk for 1 hour before incubating with Anti-ABCG8 antibody (sc-30111, Santa Cruz Ltd.), Anti-NPC1L1 antibody (TA309769, Origene Ltd.), and mouse anti-beta actin antibody monoclonal antibody (ZSGB-BIO Ltd.) at 4 °C overnight.

Techniques: Expressing, Binding Assay

Journal: Iranian Journal of Medical Sciences

Article Title: Effects of Saponin from Trigonella Foenum-Graecum Seeds on Dyslipidemia

doi:

Figure Lengend Snippet: The CYP7A1, ABCG8, NPC1L1, HMG-CoAR, SREBP-1c, ABCA1, and SR-BI mRNA expression in liver. Data are reported as mean±SD of eight rats carried out in duplicate. P values are obtained from the independent sample t-test between two groups (HFD group and another group). *P<0.05: Indicate significant difference from the HFD group; **P<0.01: Indicate extremely significant difference from the HFD group; ABCA1: ATP-binding cassette transporter A1; ABCG8: ATP-binding cassette transporter G8; CYP7A1: Cholesterol 7alpha-hydroxylase; HMG-CoAR: 3-hydroxy-3-methyl glutaryl coenzyme A reductase; NPC1L1: Niemann-Pick C1-Like 1; SR-BI: Scavenger receptor class B type I; SREBP-1: Sterol regulatory element-binding protein-1

Article Snippet: Membranes were cut into strips and blocked by 5% skim milk for 1 hour before incubating with Anti-ABCG8 antibody (sc-30111, Santa Cruz Ltd.), Anti-NPC1L1 antibody (TA309769, Origene Ltd.), and mouse anti-beta actin antibody monoclonal antibody (ZSGB-BIO Ltd.) at 4 °C overnight.

Techniques: Expressing, Binding Assay

Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Article Title: Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

doi: 10.18433/j3m029

Figure Lengend Snippet: Figure 1. Changes in relative NPC1L1, ABCG5, and ABCG8 mRNA levels in the duodenum, jejunum, and ileum of mice 24 h after oral PR administration (5 mg/kg and 15 mg/kg). Data are expressed as means ± standard deviation (SD; n = 4). Significant differences between control and PR-treated mice are shown (*p < 0.05 and **p < 0.01).

Article Snippet: Immunoreactive NPC1L1 proteins were detected using polyclonal NPC1L1 antibodies (NB400-128, Novus Biologicals, Littleton, CO, USA), monoclonal -actin antibodies (Acris Antibodies, Herford, Germany), and an ECL Prime Western Blotting Detection system (GE Healthcare).

Techniques: Standard Deviation, Control

Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Article Title: Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

doi: 10.18433/j3m029

Figure Lengend Snippet: Figure 2. Changes in relative NPC1L1, ABCG5, and ABCG8 mRNA levels in the duodenum of mice 6 h after oral PR administration (5 mg/kg and 15 mg/kg) or 24 h after oral EZ administration (5 mg/kg). Results are expressed as means ± SD (n = 4).

Article Snippet: Immunoreactive NPC1L1 proteins were detected using polyclonal NPC1L1 antibodies (NB400-128, Novus Biologicals, Littleton, CO, USA), monoclonal -actin antibodies (Acris Antibodies, Herford, Germany), and an ECL Prime Western Blotting Detection system (GE Healthcare).

Techniques:

Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Article Title: Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

doi: 10.18433/j3m029

Figure Lengend Snippet: Figure 3 The effects of PR on NPC1L1, ABCG5, and ABCG8 expression in HepG2 cells. (a) Relative NPC1L1, ABCG5, and ABCG8 mRNA levels 24 h after PR (3, 10, 30, and 300 M) treatment. (b) Time course of relative NPC1L1 mRNA expression changes after PR (30 M) treatment. (c) NPC1L1 protein expression after PR (3, 10, 30, and 300 M) treatment. Results are expressed as means ± SD (n = 4). Significant differences between (a) 0 h (control) and each time, (b), and (c) no treatment (control) and PR-treated HepG2 cells are shown (*p < 0.05, **p < 0.01, and ***p < 0.001).

Article Snippet: Immunoreactive NPC1L1 proteins were detected using polyclonal NPC1L1 antibodies (NB400-128, Novus Biologicals, Littleton, CO, USA), monoclonal -actin antibodies (Acris Antibodies, Herford, Germany), and an ECL Prime Western Blotting Detection system (GE Healthcare).

Techniques: Expressing, Control

Journal: Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques

Article Title: Pravastatin Modulate Niemann-Pick C1-Like 1 and ATP-Binding Cassette G5 and G8 to Influence Intestinal Cholesterol Absorption.

doi: 10.18433/j3m029

Figure Lengend Snippet: Figure 4 Effects of EZ on relative NPC1L1, ABCG5, and ABCG8 mRNA expression 24 h after EZ (3, 10, 30, and 300 M) treatment in HepG2 cells. Results are expressed as means ± SD (n = 4).

Article Snippet: Immunoreactive NPC1L1 proteins were detected using polyclonal NPC1L1 antibodies (NB400-128, Novus Biologicals, Littleton, CO, USA), monoclonal -actin antibodies (Acris Antibodies, Herford, Germany), and an ECL Prime Western Blotting Detection system (GE Healthcare).

Techniques: Expressing